KAPA mRNA HyperPrep Kit from Roche

Product Specs
ItemKAPA mRNA HyperPrep Kit
CompanyRoche
Price Supplier Page View Company Product Page
Catalog Number08098115702
Quantity24 libraries

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Roche

Roche Diagnostics Corporation P.O. Box 50414 9115 Hague Road

Indianapolis, Indiana

United States

Phone: +1-800-262-1640
Fax: +1-800-428-2883
Technical Service Phone: +1-800-262-4911

Company Profile

Website: sequencing.roche.com

(1) Sis2 regulates yeast replicative lifespan in a dose-dependent mannerNature CommunicationsNovember 27, 2023Tolga T. Ölmez, David F. Moreno, Ping Liu, Zane M. Johnson, Madeline M. McGinnis, Benjamin P. Tu, Mark Hochstrasser, Murat Acar#M5311AA). Stranded RNA-seq libraries were prepared with the KAPA mRNA HyperPrep Kit (Roche, #KK8580-08098115702) based on polyA capture, following the manufacturer’s instructions. The libraries were pooled for multiplexing

(2) Cephalopod retinal development shows vertebrate-like mechanisms of neurogenesisCurrent BiologyDecember 1, 2022Francesca R. Napoli, Christina M. Daly, Stephanie Neal, Kyle J. McCulloch, Alexandra R. Zaloga, Alicia Liu, Kristen M. Koenigeliminator mini-spin column,Qiagen,Cat# 1030958 Kapa mRNA-Hyper Prep kit with Poly-A selection,Roche,Cat# 08098115702 pGEM-T Easy vector,Promega,Cat# A1360 ,, ,, Deposited data,Deposited data,Deposited data DAPT:Control

(3) Characterization of HCI-EC-23 a novel estrogen- and progesterone-responsive endometrial cancer cell lineSci RepNovember 17, 2022Craig M. Rush, Zannel Blanchard, Jacob T. Polaski, Kyle S. Osborne, Krystle Osby, Jeffery M. Vahrenkamp, Chieh-Hsiang Yang, David H. Lum, Christy R. Hagan, Kimberly K. Leslie, et al.R1055). RNA-seq libraries were generated using the KAPA Stranded mRNA HyperPrep kit (Roche, KK8580/08098115702) using 500 ng of RNA. RNA-seq libraries were pooled and sequenced on either the Illumina HiSeq 2500

(4) Antitumor immunity induced by antibody-based natural killer cell engager therapeutics armed with not-alpha IL-2 variantCell reports. MedicineOctober 18, 2022Demaria O, Gauthier L, Vetizou M, Blanchard Alvarez A, Vagne C, Habif G, Batista L, Baron W, Belaïd N, Girard-Madoux M, et al.sequencing RNA-Seq libraries were prepared from 80 ng to 200 ng of total RNA with the KAPA RNA HyperPrep kit (Roche, 08098115702). The preparation was enriched in mRNA with oligo dT magnetic beads and fragmented by incubation for

(5) Characterization of Macrophage-Tropic HIV-1 Infection of Central Nervous System Cells and the Influence of InflammationJournal of VirologySeptember 14, 2022Blaide M. Woodburn, Krishna Kanchi, Shuntai Zhou, Nicholas Colaianni, Sarah B. Joseph, Ronald Swanstromprior to library preparation. cDNA libraries were prepared using the KAPA RNA HyperPrep kit (Roche, 08098115702). Paired-end RNA sequencing was performed on a NovaSeq S4 platform at a read length of 100 bp, resulting

(6) Canonical Wnt signaling induces focal adhesion and Integrin beta-1 endocytosisiScienceApril 15, 2022Tejeda-Muñoz N, Morselli M, Moriyama Y, Sheladiya P, Pellegrini M, De Robertis EM.than 8. Libraries were constructed with the KAPA mRNA Hyper-Prep kit (Roche Sequencing, cat# KK8580/08098115702) with unique dual-indexed adapters according to the manufacturer’'s protocol. Final library QC qPCR

(7) Chromatin Accessibility Identifies Regulatory Elements Predictive of Gene Expression and Disease Outcome in Multiple MyelomaClinical Cancer ResearchJune 1, 2021Benjamin G. Barwick et al.using an Agilent BioAnalyzer. Stranded mRNA-seq libraries were made using the mRNA HyperPrep kit (Kapa 08098115702) following the manufacturer’s protocol and using custom short TruSeq compatible sequencing adapters

(8) Reverse-transcribed SARS-CoV-2 RNA can integrate into the genome of cultured human cells and can be expressed in patient-derived tissuesProceedings of the National Academy of SciencesMay 25, 2021Liguo Zhang, Alexsia Richards, M. Inmaculada Barrasa, Stephen H. Hughes, Richard A. Young, Rudolf JaenischSARS-CoV-2–infected Calu3 cells. Stranded libraries were constructed with the Kapa mRNA HyperPrep kit (Roche; 08098115702). Libraries were qPCR'ed using a KAPA qPCR library quant kit as per manufacturer’s protocol. Libraries

(9) Fattening chips: hypertrophy, feeding, and fasting of human white adipocytes in vitroLab on a chipNovember 10, 2020Pope BD, Warren CR, Dahl MO, Pizza CV, Henze DE, Sinatra NR, Gonzalez GM, Chang H, Liu Q, Glieberman AL, et al.8. Isolation of mRNA and library preparation were performed using a KAPA mRNA HyperPrep kit (Roche, 08098115702) and libraries were sequenced on an Illumina NextSeq 500 sequencer. To compare transcriptomic data from

(10) Formation of Human Neuroblastoma in Mouse-Human Neural Crest ChimerasCell stem cellApril 2, 2020Cohen MA, Zhang S, Sengupta S, Ma H, Bell GW, Horton B, Sharma B, George RE, Spranger S, Jaenisch R.Kit,QIAGEN,Cat#74104 mirVana™ miRNA Isolation Kit,ThermoFischer,Cat#AM1560 KAPA mRNA HyperPrep Kit,Roche,Cat#08098115702 SuperScript III First-Strand Synthesis SuperMix,ThermoFischer,Cat#18080400 Fast SYBR Green Master

(11) Delineating the Rules for Structural Adaptation of Membrane-Associated Proteins to Evolutionary Changes in Membrane LipidomeCurrent BiologyFebruary 3, 2020Maria Makarova, Maria Peter, Gabor Balogh, Attila Glatz, James I. MacRae, Nestor Lopez Mora, Paula Booth, Eugene Makeyev, Laszlo Vigh, Snezhana Oliferenkoultra-filters,Merck,UFC900308 RNeasy Plus Mini Kit,QIAGEN,74134 polyA KAPA mRNA Hyper_Prep,Roche,08098115702 Transcriptor First Strand cDNA Synthesis Kit,Roche,04379012001 Probe Blue Mix Lo-ROX,qPCRBIO,PB20.21-01 ,, Deposited

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KAPA mRNA HyperPrep Kit from Roche

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KAPA mRNA HyperPrep Kit from Roche

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